diff --git a/browser/farbling/brave_dark_mode_fingerprint_protection_browsertest.cc b/browser/farbling/brave_dark_mode_fingerprint_protection_browsertest.cc
index 37b88677c7b..e55e7591f61 100644
--- a/browser/farbling/brave_dark_mode_fingerprint_protection_browsertest.cc
+++ b/browser/farbling/brave_dark_mode_fingerprint_protection_browsertest.cc
@@ -30,7 +30,7 @@
 #include "ui/color/color_provider_key.h"
 #include "ui/color/color_provider_manager.h"
 #include "ui/color/color_provider_source.h"
-#include "ui/native_theme/test_native_theme.h"
+#include "ui/native_theme/mock_os_settings_provider.h"
 
 using brave_shields::ControlType;
 using brave_shields::features::kBraveDarkModeBlock;
@@ -46,19 +46,6 @@ class BraveDarkModeFingerprintProtectionTest : public InProcessBrowserTest {
         brave_shields::features::kBraveShowStrictFingerprintingMode);
   }
 
-  class BraveContentBrowserClientWithWebTheme
-      : public BraveContentBrowserClient {
-   public:
-    explicit BraveContentBrowserClientWithWebTheme(const ui::NativeTheme* theme)
-        : theme_(theme) {}
-
-   protected:
-    const ui::NativeTheme* GetWebTheme() const override { return theme_; }
-
-   private:
-    const raw_ptr<const ui::NativeTheme, DanglingUntriaged> theme_;
-  };
-
   class MockColorProviderSource : public ui::ColorProviderSource {
    public:
     explicit MockColorProviderSource(bool is_dark) {
@@ -95,9 +82,6 @@ class BraveDarkModeFingerprintProtectionTest : public InProcessBrowserTest {
   void SetUpOnMainThread() override {
     InProcessBrowserTest::SetUpOnMainThread();
 
-    content::SetBrowserClientForTesting(
-        new BraveContentBrowserClientWithWebTheme(&test_theme_));
-
     host_resolver()->AddRule("*", "127.0.0.1");
     content::SetupCrossSiteRedirector(embedded_test_server());
 
@@ -135,7 +119,7 @@ class BraveDarkModeFingerprintProtectionTest : public InProcessBrowserTest {
   }
 
   void SetDarkMode(bool dark_mode) {
-    test_theme_.SetPreferredColorScheme(
+    os_settings_provider_.SetPreferredColorScheme(
         dark_mode ? ui::NativeTheme::PreferredColorScheme::kDark
                   : ui::NativeTheme::PreferredColorScheme::kLight);
     browser()
@@ -175,7 +159,7 @@ class BraveDarkModeFingerprintProtectionTest : public InProcessBrowserTest {
   }
 
  protected:
-  ui::TestNativeTheme test_theme_;
+  ui::MockOsSettingsProvider os_settings_provider_;
   MockColorProviderSource dark_color_provider_source_{true};
   MockColorProviderSource light_color_provider_source_{false};
 
diff --git a/chromium_src/ui/native_theme/native_theme_win.cc b/chromium_src/ui/native_theme/native_theme_win.cc
index 8e7f5b38925..d2101257073 100644
--- a/chromium_src/ui/native_theme/native_theme_win.cc
+++ b/chromium_src/ui/native_theme/native_theme_win.cc
@@ -7,13 +7,7 @@ namespace {
 bool s_ignore_system_dark_mode_change = false;
 }  // namespace
 
-#define BRAVE_NATIVETHEMEWIN_UPDATEDARKMODESTATUS \
-  if (s_ignore_system_dark_mode_change) {         \
-    return;                                       \
-  }
-
 #include <ui/native_theme/native_theme_win.cc>
-#undef BRAVE_NATIVETHEMEWIN_UPDATEDARKMODESTATUS
 
 namespace ui {
 
diff --git a/patches/ui-native_theme-native_theme_win.cc.patch b/patches/ui-native_theme-native_theme_win.cc.patch
deleted file mode 100644
index 80d38ce639c..00000000000
--- a/patches/ui-native_theme-native_theme_win.cc.patch
+++ /dev/null
@@ -1,12 +0,0 @@
-diff --git a/ui/native_theme/native_theme_win.cc b/ui/native_theme/native_theme_win.cc
-index 7468404c730c68bc8ba7565bf613aa69f13e3095..544b547ef365ad2eb8dc8c40e943bbaa3ad696ba 100644
---- a/ui/native_theme/native_theme_win.cc
-+++ b/ui/native_theme/native_theme_win.cc
-@@ -1669,6 +1669,7 @@ void NativeThemeWin::UpdateDarkModeStatus() {
-                                     &apps_use_light_theme);
-     in_dark_mode_ = (apps_use_light_theme == 0);
-   }
-+  BRAVE_NATIVETHEMEWIN_UPDATEDARKMODESTATUS
-   set_preferred_color_scheme(CalculatePreferredColorScheme());
-   CloseHandlesInternal();
-   NotifyOnNativeThemeUpdated();