diff --git a/browser/brave_profile_prefs.cc b/browser/brave_profile_prefs.cc
index 1911e817723..e0f72bee202 100644
--- a/browser/brave_profile_prefs.cc
+++ b/browser/brave_profile_prefs.cc
@@ -328,10 +328,6 @@ void RegisterProfilePrefs(user_prefs::PrefRegistrySyncable* registry) {
   registry->SetDefaultPrefValue(prefs::kPrivacySandboxApisEnabledV2,
                                 base::Value(false));
 
-  // Disable Chromium's privacy sandbox
-  registry->SetDefaultPrefValue(prefs::kPrivacySandboxFlocEnabled,
-                                base::Value(false));
-
   // Importer: selected data types
   registry->RegisterBooleanPref(kImportDialogExtensions, true);
   registry->RegisterBooleanPref(kImportDialogPayments, true);
diff --git a/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc b/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc
index 21b9cf2b3d4..a137d3f120b 100644
--- a/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc
+++ b/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc
@@ -520,18 +520,14 @@ TEST_F(PrivacySandboxSettingsTest, IsTopicsAllowed) {
       /*managed_cookie_setting=*/privacy_sandbox_test_util::kNoSetting,
       /*managed_cookie_exceptions=*/{});
 
-  // FLoC should be disabled since the privacy sandbox APIs can't be enabled.
-  prefs()->SetBoolean(prefs::kPrivacySandboxFlocEnabled, true);
   EXPECT_FALSE(privacy_sandbox_settings()->IsTopicsAllowed());
 
-  // Check that even bypassing PrivacySandboxSettings::SetFlocPrefEnabled,
-  // and manually updating the preferences, we still don't get this enabled.
+  // Check that even manually updating the preferences, we still don't get this
+  // enabled.
   profile()->GetTestingPrefService()->SetBoolean(
       prefs::kPrivacySandboxApisEnabled, true);
   profile()->GetTestingPrefService()->SetBoolean(
       prefs::kPrivacySandboxApisEnabledV2, true);
-  profile()->GetTestingPrefService()->SetBoolean(
-      prefs::kPrivacySandboxFlocEnabled, true);
   EXPECT_FALSE(privacy_sandbox_settings()->IsTopicsAllowed());
 
   privacy_sandbox_test_util::SetupTestState(
@@ -542,7 +538,10 @@ TEST_F(PrivacySandboxSettingsTest, IsTopicsAllowed) {
       /*user_cookie_exceptions=*/{},
       /*managed_cookie_setting=*/privacy_sandbox_test_util::kNoSetting,
       /*managed_cookie_exceptions=*/{});
-  prefs()->SetBoolean(prefs::kPrivacySandboxFlocEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabledV2, true);
   EXPECT_FALSE(privacy_sandbox_settings()->IsTopicsAllowed());
 
   privacy_sandbox_test_util::SetupTestState(
@@ -553,7 +552,10 @@ TEST_F(PrivacySandboxSettingsTest, IsTopicsAllowed) {
       /*user_cookie_exceptions=*/{},
       /*managed_cookie_setting=*/privacy_sandbox_test_util::kNoSetting,
       /*managed_cookie_exceptions=*/{});
-  prefs()->SetBoolean(prefs::kPrivacySandboxFlocEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabledV2, true);
   EXPECT_FALSE(privacy_sandbox_settings()->IsTopicsAllowed());
 
   privacy_sandbox_test_util::SetupTestState(
@@ -564,7 +566,10 @@ TEST_F(PrivacySandboxSettingsTest, IsTopicsAllowed) {
       /*user_cookie_exceptions=*/{},
       /*managed_cookie_setting=*/privacy_sandbox_test_util::kNoSetting,
       /*managed_cookie_exceptions=*/{});
-  prefs()->SetBoolean(prefs::kPrivacySandboxFlocEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabledV2, true);
   EXPECT_FALSE(privacy_sandbox_settings()->IsTopicsAllowed());
 }
 class PrivacySandboxSettingsTestCookiesClearOnExitTurnedOff
diff --git a/components/privacy_sandbox/brave_privacy_sandbox_settings.cc b/components/privacy_sandbox/brave_privacy_sandbox_settings.cc
index 6b4205d2a24..3f5924ed22b 100644
--- a/components/privacy_sandbox/brave_privacy_sandbox_settings.cc
+++ b/components/privacy_sandbox/brave_privacy_sandbox_settings.cc
@@ -25,7 +25,7 @@ BravePrivacySandboxSettings::BravePrivacySandboxSettings(
                                               pref_service,
                                               incognito_profile),
       pref_service_(pref_service) {
-  // Register observers for the Privacy Sandbox & FLoC preferences.
+  // Register observers for the Privacy Sandbox.
   user_prefs_registrar_.Init(pref_service_);
   user_prefs_registrar_.Add(
       prefs::kPrivacySandboxApisEnabled,
@@ -37,11 +37,6 @@ BravePrivacySandboxSettings::BravePrivacySandboxSettings(
       base::BindRepeating(
           &BravePrivacySandboxSettings::OnPrivacySandboxPrefChanged,
           base::Unretained(this)));
-  user_prefs_registrar_.Add(
-      prefs::kPrivacySandboxFlocEnabled,
-      base::BindRepeating(
-          &BravePrivacySandboxSettings::OnPrivacySandboxPrefChanged,
-          base::Unretained(this)));
 }
 
 BravePrivacySandboxSettings::~BravePrivacySandboxSettings() = default;
@@ -55,7 +50,4 @@ void BravePrivacySandboxSettings::OnPrivacySandboxPrefChanged() {
   if (pref_service_->GetBoolean(prefs::kPrivacySandboxApisEnabledV2)) {
     pref_service_->SetBoolean(prefs::kPrivacySandboxApisEnabledV2, false);
   }
-  if (pref_service_->GetBoolean(prefs::kPrivacySandboxFlocEnabled)) {
-    pref_service_->SetBoolean(prefs::kPrivacySandboxFlocEnabled, false);
-  }
 }